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KMID : 1023420190190040288
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Journal of Dental Hygiene Science
2019 Volume.19 No. 4 p.288 ~ p.293
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Effects of Bamboo Salt with Sodium Fluoride on the Prevention of Dental Caries
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Lee Hye-Jin
Park A-Reum
Oh Han-Na
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Abstract
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Background: Dental caries is one of several prevalent oral diseases caused by dental plaque biofilms. This study evaluated the anti-cariogenic effects of a bamboo salt (BS) and sodium fluoride (NaF) mixture on oral bacteria.
Methods: The effects of several mixtures of NaF and BS on acid production, growth, and adhesion to glass beads of Streptococcus mutans, and their anti-cariogenic properties were investigated. The growth of S. mutans was measured according to optical density at 3, 6, 9, 12, 15, 18, and 24 hours after treatment using spectrophotometry at a wavelength of 600 nm, while pH was measured using a pH meter. Adhesion of S. mutans was measured according to the weight of glass beads from each group before and after incubation. Gene expression was measured using real-time polymerase chain reaction. Acid production and growth patterns of S. mutans were compared using repeated measures analysis of variance, followed by Scheffe’s post-hoc test. The Kruskal?Wallis test was used to compare adhesion, followed by the Mann?Whitney test. Gene expression in the experimental and control samples was compared using the Student’s t-test.
Results: Growth, acid production, and adhesion of S. mutans were inhibited in all experimental groups. Expression of gft and fructosyltransferase in S. mutans was inhibited in all groups. A mixture of NaF and BS significantly reduced growth, acid production, adhesion, and gene expression of S. mutans compared with the other groups.
Conclusion: Results of the present study demonstrated that a mixture of NaF and BS was useful as a mouth rinse in preventing dental caries.
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KEYWORD
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Bamboo salt, Dental caries, Sodium fluoride, Streptococcus mutans
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